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Fragment isolation with Qiaquick (Qiagen)

Contributed by Dr. Alexei Gratchev


Qiaquick is very similar to QiaEx kit. It uses NaI solution for dissolving the agarose, and a spin-column with silica membrane. Works really fast - 5-10 min for the preparation, however the shearing of high molecular weight fragments is relatively strong. I observed clear fragment sharing with fragments of about 8kb.

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