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Fragment isolation with GFX (Amersham)

Contributed by Dr. Alexei Gratchev


In contrast to kits from Qiagen, this one uses a different agarose dissolving solution, which allows you to increase the size of the gel slice since you need only 300 mkl solution for 300 mg gel slice. The same conditions are used for all sizes of DNA fragment. Almost no shearing was observed in the case of a 12 kb vector! Isolation time is 5-10 min. Recovery rates as indicated in the manual: >80% for the isolation from the solution and >60% for the isolation from the gel. Additionla washing step improves the ligation efficiency.

I can really recommend this kit for any application.

\PRODUCTS\DNA\GFX kit for fragment isolation