TaqMan vs other labelled probes

[alex1792]

Since 5 years I intensively use dual labelled TaqMan probes for my qPCR. However there are many other ways to detect PCR product (mol. beacons, scorpion tail probes, 2 oligos etc.). Did anybody make a comparison of any of these new approaches with classical TaqMan?

[Selen]

I have tested Universal probes from Roche. Fortunately for one of the genes I had also regular TaqMan probe, synthesized on request. My result is totally diappointing. Most of the uniersal probes did not perform as I expected. Direct comparison of regular TaqMan with Universal probe showed much better result for TaqMan. Finally I had to order normal TaqMan probes for all genes I wanted to analyze.