12.10.2009 - plasmids, restriction enzymes, gels

• Lecture
• Protocol plasmid isolation
• Protocol restriction enzyme digestion
• Protocol gel electrophoresis
• map pCDH-EF1-MCS-T2A-copGFP_CD521A
• fragments pCDH-EF1-MCS-T2A-copGFP_CD521A
• map pGreenPuro (pSIH1_H1_copGFP_T2A
• fragments pGreenPuro (pSIH1_H1_copGFP_T2A
• map pPACKH1-GAG_LV500A-1
• fragments pPACKH1-GAG_LV500A-1
• map pSIH1-H1-copGFP
• fragments pSIH1-H1-copGFP

13.10.2009 - Genomic DNA and RNA

• Lecture
• Protocol isolation of genomic DNA
• Protocol isolation of total RNA

14.10.2009 - Real-time PCR

• Lecture
• Protocol real-time RT-PCR
• Results diagram
• Results serial dilutions 1
• Results serial dilutions 2

15.10.2009 - Protein analytics, IHC

• Lecture
• Protocol immunohistochemistry
• Figure 1
• Figure 2
• Figure 3
• Figure 4

16.10.2009 - Flow cytometry

• Lecture
• Protocol FACS staining of PBMCs
• FACS results

Exam

• Coming soon