|Cell culture basics|
Seven rules of a cell culturist
Preparation of Culture Media
Culture media used for the culture of monocytes/macrophages (X-vivo 10, Cambrex) is a ready to use media and may be stored at 4°C for several months.
Other cell culture media like DMEM, RPMI or Ham’s F12 (all from Biochrom) lack some components and has to be completed by addition of:
FCS contains complement, which must be "inactivated" prior to use. Once inactivated, the FCS should be aliquotted into 100ml flasks and stored at -20°C until use.
To inactivate FCS, incubate a 500 ml bottle at 56°C in a water bath for 30min. Never incubate longer than 1h. Aliquot immediately and freeze until use.
|\METHODS\Cell biology\cell culture basics|
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